5CN8
Ultrafast dynamics in myoglobin: 0.3 ps time delay
Experimental procedure
| Experimental method | LAUE |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-06-16 |
| Detector | CS-PAD CXI-2 |
| Wavelength(s) | 1.8-1.85 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.600, 28.800, 35.600 |
| Unit cell angles | 90.00, 106.50, 90.00 |
Refinement procedure
| Resolution | 14.708 - 1.800 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.21530 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5cmv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.007 |
| Data reduction software | CrystFEL |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 11719 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 148 | 74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 3.1 M ammonium sulfate |
