5CMY
Crystal structure of yeast Ent5 N-terminal domain-native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 |
| Unit cell lengths | 55.142, 55.142, 104.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.930 - 2.090 |
| R-factor | 0.18213 |
| Rwork | 0.180 |
| R-free | 0.21336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cmw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.090 |
| Number of reflections | 18365 |
| <I/σ(I)> | 27.2 |
| Completeness [%] | 99.9 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | 0.5 M Potassium thiocyanate, 0.1M BIS-TRIS propane pH 7.0 |
