5CMU
Artificial HIV fusion inhibitor AP1 fused to the C-terminus of gp41 NHR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.617, 58.811, 51.439 |
| Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
| Resolution | 31.030 - 2.113 |
| R-factor | 0.2147 |
| Rwork | 0.211 |
| R-free | 0.24400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.030 |
| High resolution limit [Å] | 2.100 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 99.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-HCl pH 8.5, 32% (w/v) PEG 3350, 0.2M MgCl2 |
