5CMP
human FLRT3 LRR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.96638 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.140, 106.581, 84.152 |
| Unit cell angles | 90.00, 90.31, 90.00 |
Refinement procedure
| Resolution | 45.022 - 2.601 |
| R-factor | 0.2358 |
| Rwork | 0.232 |
| R-free | 0.30030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.144 | 0.640 |
| Number of reflections | 39415 | |
| <I/σ(I)> | 8.765 | 1.647 |
| Completeness [%] | 95.7 | 73.1 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 295 | 0.1M Tris pH7, 50% (v/v) PEG200 |
