5CM0
Crystal structure of branched-chain aminotransferase from thermophilic archaea Geoglobus acetivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.650, 117.650, 135.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.890 - 1.900 |
| R-factor | 0.1533 |
| Rwork | 0.151 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eiy |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.830 | 2.000 | |
| High resolution limit [Å] | 1.900 | 80.000 | 1.900 |
| Rmerge | 0.066 | 0.771 | |
| Rmeas | 0.068 | 0.792 | |
| Total number of observations | 1696134 | ||
| Number of reflections | 85638 | 11949 | |
| <I/σ(I)> | 31.75 | 4.02 | |
| Completeness [%] | 99.6 | 98.6 | |
| Redundancy | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M CaCl2, 0.1M Na acetate, 20% isopropanol |
