5CLQ
Ran Y39A in complex with GPPNHP and RanBD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0039 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.530, 170.920, 135.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.984 - 3.200 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.005 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.924 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.094 | 0.871 |
| Number of reflections | 19912 | |
| <I/σ(I)> | 16.99 | 2.97 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 6.56 | 6.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.8M ammonium sulfate, 2.5% PEG 1500, 100 mM HEPES pH 7.5 |
