5CLL
Truncated Ran wild type in complex with GDP-BeF and RanBD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.600, 55.780, 122.930 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 46.806 - 2.450 |
| R-factor | 0.23653 |
| Rwork | 0.235 |
| R-free | 0.27225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.806 | 2.510 |
| High resolution limit [Å] | 2.446 | 2.446 |
| Rmerge | 0.082 | 0.291 |
| Number of reflections | 24587 | |
| <I/σ(I)> | 10.17 | |
| Completeness [%] | 95.9 | 75.3 |
| Redundancy | 3.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 291 | 18 % PEG 4000, 250 mM ammonium sulfate, 100 mM MES pH 6.25, 1 mM BeF |
