5CKK
Crystal structure of 9DB1* deoxyribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.155, 83.155, 55.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.446 - 2.801 |
| R-factor | 0.2755 |
| Rwork | 0.275 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cki |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.659 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.580 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 6.130 |
| Number of reflections | 5146 | |
| <I/σ(I)> | 33.87 | 0.43 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 4 | 25.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MPD, spermine, magnesium chloride, sodium chloride, sodium cacodylate |
