5CKD
E. coli MazF E24A form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980110 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.858, 32.462, 85.263 |
| Unit cell angles | 90.00, 98.79, 90.00 |
Refinement procedure
| Resolution | 30.357 - 1.698 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.360 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.566 | |
| Number of reflections | 20637 | |
| <I/σ(I)> | 16.7 | 2.8 |
| Completeness [%] | 97.9 | 97.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1M Tris-HCl pH 8.5, 0.2M Litium Sulfate, 30% PEG4000 |
