5CK5

Signal recognition particle receptor SRb-GDP-Mg from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.99
Spacegroup name	P 1 21 1
Unit cell lengths	64.100, 137.970, 64.310
Unit cell angles	90.00, 93.12, 90.00

Refinement procedure

Resolution	64.200 - 2.400
R-factor	0.1909
Rwork	0.186
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ck4
RMSD bond length	0.011
RMSD bond angle	1.408
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.200	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.065	0.310
Number of reflections	44980
<I/σ(I)>	6.1	2.2
Completeness [%]	99.5	99.5
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	0.2 M calcium acetate, 40% (w/v) PEG600, 0.1 M sodium cacodylate