5CK3

Signal recognition particle receptor SRb-GTP/SRX complex from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99
Spacegroup name	P 1 21 1
Unit cell lengths	88.277, 83.609, 90.213
Unit cell angles	90.00, 105.23, 90.00

Refinement procedure

Resolution	45.467 - 3.200
R-factor	0.2775
Rwork	0.276
R-free	0.29960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fh5
RMSD bond length	0.007
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.500
High resolution limit [Å]	3.200
Rmerge	0.140
Number of reflections	21109
<I/σ(I)>	7.2
Completeness [%]	99.9
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291.15	20% (w/v) PEG3350 and 0.1 M sodium citrate