5CJ3

Crystal structure of the zorbamycin binding protein (ZbmA) from Streptomyces flavoviridis with zorbamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	40.737, 78.989, 79.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.522 - 1.650
R-factor	0.1784
Rwork	0.176
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iag
RMSD bond length	0.013
RMSD bond angle	1.788
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.650	5.640	1.650
Rmerge	0.057	0.044	0.374
Rmeas	0.061	0.047	0.413
Rpim	0.021	0.016	0.170
Total number of observations	234170
Number of reflections	29473
<I/σ(I)>	14.1
Completeness [%]	93.0	95.7	79.8
Redundancy	7.9	7.4	5.6
CC(1/2)		0.997	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	289	0.1M Sodium Phosphate-citrate pH 4.2, 40% Ethanol 5 % PEG1000