5CJ1
Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.085, 64.466, 93.594 |
| Unit cell angles | 70.53, 77.51, 74.15 |
Refinement procedure
| Resolution | 48.679 - 2.100 |
| R-factor | 0.2096 |
| Rwork | 0.207 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.891 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.430 |
| Number of reflections | 67161 | |
| <I/σ(I)> | 15.57 | 2.3 |
| Completeness [%] | 97.6 | 96.3 |
| Redundancy | 3.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5 |
