5CIW
Ran GDP Y39A mutant monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.900, 59.330, 64.790 |
| Unit cell angles | 90.00, 96.55, 90.00 |
Refinement procedure
| Resolution | 19.890 - 1.750 |
| R-factor | 0.20115 |
| Rwork | 0.199 |
| R-free | 0.24636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human Ran GDP |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.164 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.891 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.034 | 0.275 |
| Number of reflections | 41838 | |
| <I/σ(I)> | 23.42 | |
| Completeness [%] | 94.7 | 80.3 |
| Redundancy | 3.64 | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 34 % PEG 1000, 50 mM HEPES pH 7.2, 20 mM MgCl2, 1 mM BeF |
