5CIV
Sibling Lethal Factor Precursor - DfsB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2011-05-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.590, 47.680, 72.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.509 - 1.384 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.19880 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.022 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.510 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.025 | |
| Number of reflections | 32427 | |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 94.7 | 74.2 |
| Redundancy | 6.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 12% PEG 550 MME, 6% PEG 20,000, 0.1 M Sodium formate; 0.1 M Ammonium acetate; 0.1 M Sodium citrate tribasic dihydrate; 0.1 M Sodium potassium tartrate tetrahydrate; 0.1 M Sodium oxamate, 0.1 M Tris, 0.1 M Bicine |
