5CIK
Crystal Structure of Xaa-Pro dipeptidase from Xanthomonas campestris in citrate condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.179, 103.673, 136.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.250 - 2.200 |
| R-factor | 0.14957 |
| Rwork | 0.148 |
| R-free | 0.18134 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fkc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.627 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.099 |
| Number of reflections | 42175 | |
| <I/σ(I)> | 43.6 | 20.8 |
| Completeness [%] | 98.9 | 91.2 |
| Redundancy | 12.3 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 294 | 0.1 M Citric acid, 20% PEG6000 |
