5CIG
Complex of yeast cytochrome c peroxidase (W191G) with iso-1 cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.380, 117.109, 88.929 |
| Unit cell angles | 90.00, 105.06, 90.00 |
Refinement procedure
| Resolution | 29.179 - 2.060 |
| R-factor | 0.2825 |
| Rwork | 0.282 |
| R-free | 0.29780 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.810 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
| Rmerge | 0.121 | 0.074 | 0.460 |
| Total number of observations | 152557 | ||
| Number of reflections | 52508 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 96.1 | 90.2 | 98.3 |
| Redundancy | 2.9 | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG 3350 15-15%, 100 mM NaAcetate, 175 mM NaCl |
