5CID
Complex of yeast cytochrome c peroxidase (W191G) bound to o-toluidine with iso-1 cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.380, 111.906, 87.894 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 42.580 - 2.763 |
| R-factor | 0.2197 |
| Rwork | 0.214 |
| R-free | 0.26820 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.879 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.092 | 0.042 | 0.388 |
| Total number of observations | 109129 | ||
| Number of reflections | 20376 | ||
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 93.5 | 99.8 | 65.7 |
| Redundancy | 5.4 | 5.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 3350 15-15%, 100 mM NaAcetate, 175 mM NaCl |
