5CIB
Complex of yeast cytochrome c peroxidase (W191G) bound to 2,4-dimethylaniline with iso-1 cytochrome c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9778 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.371, 107.461, 87.217 |
Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
Resolution | 39.298 - 3.011 |
R-factor | 0.2424 |
Rwork | 0.236 |
R-free | 0.30390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.826 |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 15317 |
<I/σ(I)> | 17.2 |
Completeness [%] | 95.9 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350 15-15%, 100 mM NaAcetate, 175 mM NaCl |