5CHY

STRUCTURE OF CHEMOTAXIS PROTEIN CHEY

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	292
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	45.600, 46.550, 53.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.185 *
Rwork	0.185
Structure solution method	PHASE WITH WILD TYPE
Starting model (for MR)	MODIFIED WITH WILD-TYPE CHEY
RMSD bond length	0.012
RMSD bond angle	0.044
Data reduction software	RIGAKU
Data scaling software	RIGAKU
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.066
Total number of observations	21022 *
Number of reflections	7433
<I/σ(I)>	1
Completeness [%]	92.0	80.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	4 *	28% PEG 8000, 0.12 M CALCIUM ACETATE, 0.1 M CACODYLATE BUFFER, PH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	28 (%)
2	1	reservoir	calcium acetate	0.12 (M)
3	1	reservoir	cacodylate	0.1 (M)