5CHY

STRUCTURE OF CHEMOTAXIS PROTEIN CHEY

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	292
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	45.600, 46.550, 53.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.185 *
Rwork	0.185
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.044
Data reduction software	RIGAKU
Data scaling software	RIGAKU
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.066
Total number of observations	21022 *
Number of reflections	7433
<I/σ(I)>	1
Completeness [%]	92.0	80.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	4 *	28% PEG 8000, 0.12 M CALCIUM ACETATE, 0.1 M CACODYLATE BUFFER, PH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	28 (%)
2	1	reservoir	calcium acetate	0.12 (M)
3	1	reservoir	cacodylate	0.1 (M)