5CHS

N-terminal domain of the vesicular stomatitis virus L protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-04
Detector	RAYONIX MX300HS
Wavelength(s)	0.97932
Spacegroup name	P 21 21 21
Unit cell lengths	64.793, 77.237, 136.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.242 - 1.800
R-factor	0.1744
Rwork	0.173
R-free	0.19980
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.958
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.066	0.045	0.416
Rmeas	0.073	0.049	0.459
Rpim	0.030	0.020	0.189
Total number of observations	368628
Number of reflections	64013
<I/σ(I)>	9.9
Completeness [%]	99.5	96.4	99.8
Redundancy	5.8	5.4	5.7
CC(1/2)		0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	100mM Tris, pH 8.0, 1M NH4Formate, 4% Jeffamine T403 with 0.05% B-octylglucopyranoside