5CHL

Structural basis of H2A.Z recognition by YL1 histone chaperone component of SRCAP/SWR1 chromatin remodeling complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77
Detector technology	IMAGE PLATE
Collection date	2013-07-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	108.047, 108.047, 58.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.967 - 1.892
R-factor	0.2018
Rwork	0.200
R-free	0.23130
RMSD bond length	0.010
RMSD bond angle	1.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.892
Number of reflections	28027
<I/σ(I)>	23.3
Completeness [%]	99.9
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.19 mM CYMAL-7, 0.1 M HEPES, pH 7.5, 40% Polyethylene glycol 400