5CG0

Crystal structure of Spodoptera frugiperda Beta-glycosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54187
Spacegroup name	P 1
Unit cell lengths	53.825, 64.994, 257.189
Unit cell angles	93.16, 92.04, 112.18

Refinement procedure

Resolution	49.750 - 2.090
R-factor	0.1952
Rwork	0.192
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vif
RMSD bond length	0.008
RMSD bond angle	1.208
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.080	0.052	0.539
Rmeas	0.095	0.061	0.650
Rpim	0.050	0.031	0.356
Total number of observations	566584
Number of reflections	173155
<I/σ(I)>	12.1
Completeness [%]	91.4	99	90
Redundancy	3.3	3.9	3.2
CC(1/2)		0.996	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	291	100 mM Bis-Tris-HCl, 100 mM NaCL, 22% (w/v) PEG 3350