5CES

C-terminal domain of the R-type pyocin baseplate protein PA0618

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91963
Spacegroup name	P 43
Unit cell lengths	72.611, 72.611, 46.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.170 - 2.102
R-factor	0.192
Rwork	0.190
R-free	0.23600
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.705
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.170
High resolution limit [Å]	2.102
Number of reflections	27538
<I/σ(I)>	30.41
Completeness [%]	99.6
Redundancy	5.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 5000 MME, ammonium acetate, MES