5CEJ
The crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Yersinia pestis at 2.50A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.7108 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 88.160, 88.160, 54.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.169 - 2.500 |
| R-factor | 0.2215 |
| Rwork | 0.220 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.849 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1834) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.170 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7814 | |
| <I/σ(I)> | 9.7 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 300 mM ammonium phosphate monobasic, trimethylamine N-oxide dihydrate |
