5CEG

X-ray structure of toxin/anti-toxin complex from Mesorhizobium opportunistum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	I 2 2 2
Unit cell lengths	43.180, 118.840, 211.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.590
R-factor	0.1692
Rwork	0.169
R-free	0.19540
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.068
Data reduction software	xia2 (0.3.6.3)
Data scaling software	xia2 (0.3.6.3)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.300	1.630
High resolution limit [Å]	1.590	1.590
Rmerge	0.098
Number of reflections	73683
<I/σ(I)>	13.5
Completeness [%]	100.0
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	400 mM Sodium Acetate, 100 mM Sodium Citrate pH5.5, 20% PEG 4000, 20 % glycerol