5CEF
Cystal structure of aspartate semialdehyde dehydrogenase from Cryptococcus neoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.590, 130.960, 91.940 |
| Unit cell angles | 90.00, 92.87, 90.00 |
Refinement procedure
| Resolution | 66.050 - 2.600 |
| R-factor | 0.1831 |
| Rwork | 0.181 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hsk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.513 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.050 | 2.680 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.415 |
| Number of reflections | 55730 | |
| <I/σ(I)> | 4.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG8000, Ethylene glycol |
