5CE6

N-terminal domain of FACT complex subunit SPT16 from Cicer arietinum (chickpea)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	P 1 21 1
Unit cell lengths	46.019, 69.112, 71.587
Unit cell angles	90.00, 102.92, 90.00

Refinement procedure

Resolution	34.888 - 1.700
R-factor	0.1818
Rwork	0.180
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cb6
RMSD bond length	0.006
RMSD bond angle	0.976
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.072	0.796
Number of reflections	47708
<I/σ(I)>	24.3	2
Completeness [%]	99.0	95.6
Redundancy	6.5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	294	50 mM sodium acetate, pH 4.2, 200 mM K acetate, 10 mM MgCl2, 15 mM CaCl2, 34% PEG 3350, 5% glycerol, 10 mM Tris-HCl, 100 mM NaCl