5CE0
Crystal structure of conserpin with Z-mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.220, 150.270, 51.015 |
| Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
| Resolution | 37.568 - 2.300 |
| R-factor | 0.2027 |
| Rwork | 0.201 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cdx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.570 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.147 |
| Number of reflections | 32616 |
| <I/σ(I)> | 30.83 |
| Completeness [%] | 99.6 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | Crystals appeared within 5 days in 20% (v/v) polyethylene glycol (PEG) 3350, 0.01 M Bis Tris and 0.2 M Magnesium chloride (hexahydrate) |
