5CDY

The crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Yersinia pestis at 2.85A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.7108
Spacegroup name	P 1 21 1
Unit cell lengths	64.740, 96.850, 71.550
Unit cell angles	90.00, 104.91, 90.00

Refinement procedure

Resolution	29.766 - 2.850
R-factor	0.2135
Rwork	0.212
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.622
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.150	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	19603
<I/σ(I)>	9.4	2
Completeness [%]	98.1	98
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	300 mM ammonium phosphate monobasic, 100 mM sodium bromide