5CD9
Crystal structure of the CTD of Drosophila Oskar protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.999, 68.999, 100.687 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.637 - 2.102 |
| R-factor | 0.1633 |
| Rwork | 0.161 |
| R-free | 0.20340 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.934 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.115 | 0.062 | 0.423 |
| Total number of observations | 64051 | ||
| Number of reflections | 16668 | ||
| <I/σ(I)> | 8.7 | 3 | |
| Completeness [%] | 99.8 | 99.5 | 99.9 |
| Redundancy | 3.8 | 3.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 800mM (NH4)2SO4, 200mM LiSO4, 100mM NaAC pH4.6 |
