5CCO
Staphylococcus bacteriophage 80alpha dUTPase with dUMP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 87.000, 87.000, 87.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.520 - 2.330 |
| R-factor | 0.20322 |
| Rwork | 0.201 |
| R-free | 0.24437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zez |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.520 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.100 | |
| Number of reflections | 9645 | |
| <I/σ(I)> | 16.2 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 60% ethanol, 0.1 M NaCl |
