5CCK

Crystal structure of Human anti-HIV-1 broadly neutralizing antibody 3BC315 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	48.075, 49.522, 159.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.280 - 1.950
R-factor	0.1597
Rwork	0.156
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.182
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.280
High resolution limit [Å]	1.950
Number of reflections	27626
<I/σ(I)>	61.2
Completeness [%]	96.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.3	298	0.1M CAPS pH 10.3, 0.25 M NaCl, 20% PEG8000