5CCF

Structure of Mouse ADP-Dependent Glucokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	45.923, 58.686, 160.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.400 - 2.100
R-factor	0.19557
Rwork	0.193
R-free	0.24416
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1UA4 1l2l & 1GC5
RMSD bond length	0.009
RMSD bond angle	1.273
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.416	2.160
High resolution limit [Å]	2.094	2.100
Rmerge	0.120	0.760
Number of reflections	26310
<I/σ(I)>	9.3	2
Completeness [%]	99.8	100
Redundancy	4.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M NH4Cl, 20% w/v PEG 3350