5CC0

AncSR2 - TSLP nGRE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	50.334, 72.507, 104.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.469 - 2.405
R-factor	0.2166
Rwork	0.214
R-free	0.23720
RMSD bond length	0.013
RMSD bond angle	1.619
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.400
Number of reflections	15335
<I/σ(I)>	34.5
Completeness [%]	99.8
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	in 0.1 M HEPES (pH 7.5), 10% PEG 20000, 5% glycerol, 5% ethanol