5CBY

AncGR2 DNA Binding Domain - (+)GRE Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	131.644, 38.798, 98.250
Unit cell angles	90.00, 119.65, 90.00

Refinement procedure

Resolution	36.743 - 1.997
R-factor	0.1847
Rwork	0.182
R-free	0.21700
RMSD bond length	0.010
RMSD bond angle	1.202
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	27141
<I/σ(I)>	20.9
Completeness [%]	91.3
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES (pH 7.5) and 15% PEG 8000