5CAX

CRYSTAL STRUCTURE OF METHANOSARCINA ACETIVORANS METHANOREDOXIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2013-09-15
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	59.154, 59.154, 172.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.492 - 2.451
R-factor	0.2207
Rwork	0.215
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nzn
RMSD bond length	0.011
RMSD bond angle	1.549
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.400
Number of reflections	21962
<I/σ(I)>	11.7
Completeness [%]	94.7
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES buffer, pH 7.5, 0.05 M cadmium sulfate and 1M sodium acetate