5CA8
Structures of the yeast dynamin-like GTPase Sey1p in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.120, 43.620, 97.560 |
| Unit cell angles | 90.00, 108.12, 90.00 |
Refinement procedure
| Resolution | 46.361 - 2.300 |
| R-factor | 0.2331 |
| Rwork | 0.230 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ca9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.514 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 40802 |
| <I/σ(I)> | 10.2 |
| Completeness [%] | 99.1 |
| Redundancy | 2.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 20 mM Tris pH 8.5, 160 mM Potassium sodium tartrate tetrahydrate, 4 mM GDP, 21% w/v PEG3350 |
