5CA5
Structure of the C. elegans NONO-1 homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96580 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.318, 63.115, 83.838 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 19.670 - 2.400 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.30080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wii |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.670 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.800 | |
| Number of reflections | 23755 | |
| <I/σ(I)> | 1.4 | |
| Completeness [%] | 92.4 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M HEPES, 0.1 M Ammonium sulphate, 15.0 - 20.0 % PEG 3350 |
