5C9Z
Crystal structure of PRMT5:MEP50 with EPZ015866 and sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.810, 137.780, 178.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.530 - 2.360 |
| R-factor | 0.2392 |
| Rwork | 0.237 |
| R-free | 0.28810 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.460 | 2.420 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.048 | 0.607 |
| Number of reflections | 52114 | |
| <I/σ(I)> | 18.4 | 2.3 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.2 M Sodium acetate, 0.1 M Sodium citrate pH 6.1, 10% w/v PEG 4000 |
