5C9S

Crystal Structure of the C-terminal domain of Rrp5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-05-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97530
Spacegroup name	P 32 2 1
Unit cell lengths	114.140, 114.140, 67.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.672 - 2.700
R-factor	0.2242
Rwork	0.221
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.008
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.070	57.070	2.830
High resolution limit [Å]	2.700	8.960	2.700
Rmerge	0.185	0.071	0.680
Rmeas	0.195	0.075	0.719
Rpim	0.061	0.023	0.231
Total number of observations	141367	4222	18053
Number of reflections	14309
<I/σ(I)>	7.5	13.7	3
Completeness [%]	99.9	99.6	100
Redundancy	9.9	9.6	9.5
CC(1/2)	0.993	0.996	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammuniom tartrate, 20% PEG3350