5C9S
Crystal Structure of the C-terminal domain of Rrp5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97530 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.140, 114.140, 67.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.672 - 2.700 |
| R-factor | 0.2242 |
| Rwork | 0.221 |
| R-free | 0.28260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.008 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 57.070 | 57.070 | 2.830 |
| High resolution limit [Å] | 2.700 | 8.960 | 2.700 |
| Rmerge | 0.185 | 0.071 | 0.680 |
| Rmeas | 0.195 | 0.075 | 0.719 |
| Rpim | 0.061 | 0.023 | 0.231 |
| Total number of observations | 141367 | 4222 | 18053 |
| Number of reflections | 14309 | ||
| <I/σ(I)> | 7.5 | 13.7 | 3 |
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 9.9 | 9.6 | 9.5 |
| CC(1/2) | 0.993 | 0.996 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammuniom tartrate, 20% PEG3350 |
