5C9H
Structural Basis of Template Boundary Definition in Tetrahymena Telomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.530, 117.770, 131.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.512 - 3.000 |
| R-factor | 0.2439 |
| Rwork | 0.239 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r4g |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.820 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.512 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.231 | 0.969 |
| Number of reflections | 18728 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200mM KCl, 10mM MgCl2, 50mM MES monohydrate pH 5.6, 4% PEG 8000 |
