5C9A
Crystal structure of empty coxsackievirus A16 particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 289 |
Detector technology | PIXEL |
Collection date | 2012-09-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97620 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 491.400, 491.400, 708.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.980 - 2.700 |
R-factor | 0.291 |
Rwork | 0.291 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c4w |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.535 | |
Number of reflections | 1437314 | |
<I/σ(I)> | 1.4 | 0.4 |
Completeness [%] | 62.1 | 16.2 |
Redundancy | 1.8 | 1.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 7.0 |