5C98

1.45A resolution structure of SRPN18 from Anopheles gambiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	40.419, 87.485, 194.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.692 - 1.450
R-factor	0.1661
Rwork	0.165
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pzf
RMSD bond length	0.008
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.700	48.700	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.043	0.026	0.841
Rpim	0.018	0.011	0.346
Total number of observations	808528	5327	39879
Number of reflections	122919
<I/σ(I)>	20.1	56.5	2.5
Completeness [%]	99.6	98.9	98.9
Redundancy	6.6	6	6.7
CC(1/2)	0.999	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% (w/v) PEG 3350, 100 mM Bis-Tris, 200 mM NaCl, 20% PEG 400