5C92
Novel fungal alcohol oxidase with catalytic diversity among the AA5 family, in complex with copper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.580, 81.580, 154.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.120 - 2.100 |
| R-factor | 0.14451 |
| Rwork | 0.142 |
| R-free | 0.20097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c86 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.120 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.701 |
| Number of reflections | 29704 | |
| <I/σ(I)> | 20.6 | 4.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.9 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50% tacsimate buffer pH 6.5 |
