5C87

Crystal structure of the human BRPF1 bromodomain in complex with SEED2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00003
Spacegroup name	P 32 2 1
Unit cell lengths	60.559, 60.559, 63.599
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.339 - 1.550
R-factor	0.1827
Rwork	0.182
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lc2
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.630
High resolution limit [Å]	1.550	1.550
Rmerge	0.048	0.341
Number of reflections	20025
<I/σ(I)>	26.6	4.8
Completeness [%]	100.0	100
Redundancy	9.1	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris propane, pH6.5, 0.2 M sodium nitrate, 20%PEG33500