5C7R
Revealing surface waters on an antifreeze protein by fusion protein crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.050, 110.800, 96.570 |
Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.000 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.133 | 1.362 |
Number of reflections | 72126 | |
<I/σ(I)> | 9.36 | 1.1 |
Completeness [%] | 96.7 | 86.2 |
Redundancy | 3.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 1.75 M (NH4)2SO4, 100 mM NaOAC (pH 4.4) |