5C76
ATP-driven lipid-linked oligosaccharide flippase PglK in apo-inward facing state (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.410, 183.330, 136.260 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 29.823 - 3.940 |
| R-factor | 0.2773 |
| Rwork | 0.275 |
| R-free | 0.32930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c78 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.939 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.040 | |
| High resolution limit [Å] | 3.940 | 17.620 | 3.940 |
| Rmerge | 0.094 | 0.042 | 2.036 |
| Rmeas | 0.097 | 0.043 | 2.150 |
| Total number of observations | 480068 | ||
| Number of reflections | 33489 | 364 | 292 |
| <I/σ(I)> | 12.36 | 39.52 | 1.54 |
| Completeness [%] | 86.0 | 78.8 | 10.1 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.4 | 298 | Glycine-NaOH; KCl; MgCl2; PEG600 |
