5C66
E. Coli Alkaline Phosphatase in complex with tungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 161.260, 161.260, 139.432 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.740 - 2.030 |
| R-factor | 0.19702 |
| Rwork | 0.195 |
| R-free | 0.23987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tg0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.862 |
| Data reduction software | iMOSFLM (7.1.1) |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.740 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.030 | |
| Number of reflections | 64098 | |
| <I/σ(I)> | 14.1 | 3.9 |
| Completeness [%] | 93.6 | 93.4 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | sodium MOPS, sodium chloride, zinc chloride, magnesium chloride, PEG3350, ammonium fluoride, sodium tungstate |
