5C4X
Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 220.790, 393.360, 281.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 4.000 |
| R-factor | 0.2103 |
| Rwork | 0.210 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fki |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.420 | |
| Number of reflections | 103153 | |
| <I/σ(I)> | 12.5 | 1.95 |
| Completeness [%] | 97.3 | 96.4 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG 4000, SODIUM CITRATE, MES, DTT |
